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M94A1840.TXT
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1994-10-24
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Document 1840
DOCN M94A1840
TI A new supplemental assay for the characterization and simultaneous
detection of HIV antibody and antigen.
DT 9412
AU Delagneau JF; Basse JM; Morat L; Prigent V; Sanjuan A; SANOFI
Diagnostics PASTEUR, Marnes la Coquette, France.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):44 (abstract no. 148B). Unique
Identifier : AIDSLINE ICA10/94370735
AB OBJECTIVE: To provide a highly sensitive assay by simultaneous detection
of anti ENV HIV-1 and/or HIV-2 antibody and/or GAG antigen with
characterization of each reactive analyte. METHODS: Well documented
serial bleeds of HIV-1 seroconverters were tested for p24 antigen, gp41
and HIV2 gp36 antibodies with a unitary membrane test strip containing
specific bands for each analyte to be detected. This assay, called
DECISCAN HIV Ab/Ag, is based on a sandwich ELISA format using HIV1 and
HIV2 ENV antigens (synthetic peptides and recombinant glycoprotein) for
antibody detection and monoclonal antibodies for p24 detection. RESULTS:
In all seroconverters tested, HIV-1 antibody detection bands were
reactive in the same bleeds as in 3rd generation antibody assays. In
three patients, p24 was detected in at least one bleed prior to antibody
detection. Three other seroconversions became antibody and antigen
positive in the same bleed. One seroconverters became antibody reactive
without antigen detection. No reactivity was observed in 200 HIV
antibody negative samples from normal blood donors. DISCUSSION AND
CONCLUSION: The new DECISCAN HIV Ab/Ag supplemental assay allows an
early diagnosis of HIV seroconverters based on highly sensitive anti ENV
HIV antibody detection improved by simultaneous p24 antigen detection.
This assay gives useful additonal data for patients with low antibody
reactivity or WB indeterminate results.
DE Enzyme-Linked Immunosorbent Assay/*METHODS/STATISTICS & NUMER DATA
Gene Products, env/BLOOD/IMMUNOLOGY Gene Products, gag/BLOOD/IMMUNOLOGY
Human HIV Antibodies/*BLOOD HIV Antigens/*BLOOD/IMMUNOLOGY HIV Core
Protein p24/BLOOD/IMMUNOLOGY HIV Envelope Protein gp41/BLOOD/IMMUNOLOGY
HIV Seropositivity/DIAGNOSIS/IMMUNOLOGY/MICROBIOLOGY
HIV-1/IMMUNOLOGY/ISOLATION & PURIF HIV-2/IMMUNOLOGY/ISOLATION & PURIF
Sensitivity and Specificity MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).